SASSE RUTH NYABA Iya

• Conduct animal study in Cre-recombinase knockout mice to investigate the role of a gene in the metabolism of magnesium ion in the kidney.
• Assist in animal care and dissection.
• Collection of serum, urine and feces samples from mice.
• Transfect HEK cells and conduct in-vitro experiments to determine the influence of the transfected gene/protein on magnesium uptake.
• Immunostaining of kidney tissues to access the protein concentration of the gene under study.
• Extraction of proteins, RNA and DNA from kidney tissue.
• CRISPR-cas9 gene editing.
• Measure protein, RNA and DNA concentrations by western blot and RT-PCR.
• Submitting samples to sequencing.
• Perform Cloning of Phage display hits into mammalian expression vectors
• Plan experiments and propose follow-up actions.
• Record experimental progress and data in excel sheets and electronic laboratory notebooks.
• Analyze data using SPSS, GraphPad Prism and python.
• Present results in written reports and oral presentation
• Design and execute follow-up experiments.

• Assist students with tutorials, assignments and laboratory activities and exercises.

• Slicing of frozen brain tissue.
• Immunostaining of brain tissue slices.
• Produce fluorescent images of stained brain tissue slices using confocal fluorescent microscope.
• Writing code which is used to analyze generated fluorescent images in MatLab.
• Analysis of data and presentation in a written report and oral presentation.

• Field and laboratory collection of parasitological data
• Animal studies: Animal care and infection of laboratory animals with parasites.
• Collection of blood, urine and feces samples from laboratory animals and humans.
• Analysis of blood/serum by ELISA and western blot.
• Maintain accurate records.
• Communicate results orally and in written reports.

• Teaching Biology and Human biology
• Preparing lessons, designing and administration of evaluations.

Major study aspects are evaluating the different gene regulators like transcription factors and epigenetic modifications (DNA methylation, chromatin remodelling); elucidating related regulation mechanisms and functions. Molecular biology techniques for identifying biomarkers and drug targets and methods to study disease mechanisms and the functions of biological molecules like protein and RNA.

Courses focused on studying the parasites which infest animals with emphasis on human parasites, their pathology, epidemiology and control. Parasites’ biology, and life cycle, disease mechanism, manifestation and diagnosis

Invertebrate and vertebrate anatomy and physiology

Acquisition of nutrients by vertebrates and invertebrates

Ethics and animal welfare

Legislation on animal use and animal care for mouse and rat

Minimally invasive procedures without anesthesia

Design of procedures and projects

Anesthesia for minor procedures

Principles of surgery