Rahmen Bin Ali
Barneveld
Summary
Talented Research Technician proudly offering experience applying standard protocols to research and finding ways to improve current methods.
Highly enthusiastic professional with working knowledge of scientific terminology and mathematical concepts.
Driven to be top performer. Dedicated employee driven and determined in conducting analytical procedures in laboratories.
Communicative Research Technician capable of performing experimental testing and preparing reagents.
Multitasking Research Technician promoting more than 20 years career gathering and analyzing data.
Focused individual proudly demonstrating a vast understanding of lab procedures and statistical techniques.
Methodical individual follows precise instructions and procedures in support of research.
Knowledgeable about scientific methodologies and exhibits meticulous attention to detail. Excellent observation, reporting and data management skills.
Overview
36
36
years of professional experience
1
1
Certification
Work History
Research Technician
Animal Modeling Facility
08.2002 - Current
- intra peritoneal injection of hormones during superovulation process.
- giving anesthetic & euthanastic of mice during surgical implantation of embryos by intra peritoneal & subcutaneous injection of anesthetic or pain relief respectively
- isolation of oocytes, zygotes, morulas or blastos from donor mice for microinjection, or embryos cryopreservations.
- vasectomy of mice
- making mice models for researchers, both internally or externally by zygotes or blastocysts injection.
- implantation of embryos into pseudopregnant females after microinjections by surgical implantation via the oviducts or embryo transfer (blastocysts) via the uterus or using NSET (Non Surgical Embryo Transfer) via the vagina.
- sperm and embryos cryopreservations
- sperm and embryos rederivations for researchers to bring back the mouse colony on the facility.
- giving assistance for the shipment of frozen sperm or embryos sample to overseas in dry shipper or dry ice mode.
- superovulations of donor mice for embryos productions by intra peritoneal injections of hormones.
- planning of daily work schedules for the AMF (embryo) team.
- maintaining the datas for the storage of frozen and embryo in our cryobank system.
- taking care and the maintainence of the transgenic room equipments. Making stock solutions for routine usage.
- making in-house transfer glass pipettes for daily usage.
- performing in-vitro fertilization (IVF) both for sperm quality control and sperm recovery.
- maintaining the mice colony for our embryos production, in particular the availability of the fertile males, vasectomised males, F1 females and donor females.
- filling up and maintaining the liquid nitrogen tank on the floor.
Research Technician
Animal Facilty @ IMCB
01.2000 - 03.2002
- embryos isolation both for zygote and blastocysts microinjections.
- implantations of injected embryos into pseudopregnant females.
- toe clippings for genotyping.
- husbandy work - cleaning & changing dirty mouse cages.
- superovulation of donor female mice for embryos productions.
- ordering of mice externally.
- handling of rats for antibody production for the research groups – injection of antigen & then bleeding the rat for the blood.
- production of our own vasectomized males for our in-house usage
Research Technician
Institute of Molecular & Cell Biology (IMCB)
05.1988 - 01.2000
- working in one research lab (DNA repair & chemical carcinogenesis) under the supervision of Dr. Ben Li.
- assisting the student and post-doc in their daily laboratory experiment, mostly molecular biology work like DNA sequencing on gels, running protein gels, western blotting, cell & tissue culturing and cell & tissue staining
Education
Diploma of Higher Education - Medical Laboratory Technology
Singapore Polytechnic
Singapore1988
Skills
- Data maintainence
- Microinjections
- Sperm & Embryos Cryopreservation
- Sperm & Embryo Recovery
- In-vitro Fertilization
Accomplishments
- Co authorships in several research publications including 1 first authorship during my laboratory work at IMCB under the supervision of Dr. Ben Li and another under the supervision of Dr. Ivo Huijbers at the Netherlands Cancer Institute.
- The following are some of the publication lists:
◆ Identification of an alternative triglyceride biosynthesis pathway.
McLelland GL, Lopez-Osias M, Verzijl CRC, Ellenbroek BD, Oliveira RA, Boon NJ, Dekker M, van den Hengel LG, Ali R, Janssen H, Song JY, Krimpenfort P, van Zutphen T, Jonker JW, Brummelkamp TR.Nature. 2023 Sep;621(7977):171-178.
◆ Effective CRISPR/Cas9-mediated correction of a Fanconi anemia defect by error-prone end joining or templated repair.
van de Vrugt HJ, Harmsen T, Riepsaame J, Alexantya G, van Mil SE, de Vries Y, Bin Ali R, Huijbers IJ, Dorsman JC, Wolthuis RMF, Te Riele H.Sci Rep. 2019 Jan 25;9(1):768.
◆ Modeling invasive lobular breast carcinoma by CRISPR/Cas9-mediated somatic genome editing of the mammary gland.
Annunziato S, Kas SM, Nethe M, Yücel H, Del Bravo J, Pritchard C, Bin Ali R, van Gerwen B, Siteur B, Drenth AP, Schut E, van de Ven M, Boelens MC, Klarenbeek S, Huijbers IJ, van Miltenburg MH, Jonkers J.Genes Dev. 2016 Jun 15;30(12):1470-80.
◆ Using the GEMM-ESC strategy to study gene function in mouse models.
Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J.Nat Protoc. 2015 Nov;10(11):1755-85.
◆ Selective resistance to the PARP inhibitor olaparib in a mouse model for BRCA1-deficient metaplastic breast cancer.
Henneman L, van Miltenburg MH, Michalak EM, Braumuller TM, Jaspers JE, Drenth AP, de Korte-Grimmerink R, Gogola E, Szuhai K, Schlicker A, Bin Ali R, Pritchard C, Huijbers IJ, Berns A, Rottenberg S, Jonkers J.Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8409-14.
◆ Improved pregnancy and birth rates with routine application of nonsurgical embryo transfer.
Bin Ali R, van der Ahé F, Braumuller TM, Pritchard C, Krimpenfort P, Berns A, Huijbers IJ.Transgenic Res. 2014 Aug;23(4):691-5.
◆ Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.
Huijbers IJ, Bin Ali R, Pritchard C, Cozijnsen M, Kwon MC, Proost N, Song JY, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak EM, Jonkers J, Berns A.EMBO Mol Med. 2014 Feb;6(2):212-25.
Certification
- Completed a 1 week course for endorsement to work with rodents at the Animal facility at the Netherlands Cancer Institute after consulting with the former IvD (Dr. R.G.M. ten Berg).
Course: Course on Experimental Microsurgical Techniques and Radio-Telemetry)
Date: 17-21 January 2005 - Completed successfully a 1 week course at the MRC Harwell in Oxford, UK for the Embryo Cryopreservation & in-vitro fertilization course.
Course: EMMA Cryopreservation Training Course
Date: 2-5 March 2015 - Short presentation & poster presentation @ the ISTT meeting in Edinburgh
Meeting: ISTT Meeting 2014 - Attended several transgenic symposium overseas (ISTT)
- Webinar - one of the speaker organised by ISTT, via online with the transgenic members around the world.
International Society of Transgenic Technologies (ISTT)
Topic: Non-Surgical Embryo Transfer (NSET)
Date: 23 & 30 March 2022
Timeline
Research Technician
Animal Modeling Facility
08.2002 - Current
Research Technician
Animal Facilty @ IMCB
01.2000 - 03.2002
Research Technician
Institute of Molecular & Cell Biology (IMCB)
05.1988 - 01.2000
Diploma of Higher Education - Medical Laboratory Technology
Singapore Polytechnic
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