Harika Patibandla

Effect of Pre-Existing Anti-PEG Antibodies on Innate Immunity

• Isolated Serums from patients and mice with pre-existing anti-PEG antibodies.
• Assessed C3a concentrations using human and mice C3a ELISA kits and quantified IFN-gamma in plasma samples.
• Optimized ELISA procedures and analyzed performance characteristics to validate assay sensitivity and specificity.
• Extracted bone marrow-derived dendritic cells (BMDCs) from Swiss Webster mice for in vitro stimulation assays.
• Performed flow cytometry analysis for quantitative and qualitative assessment of cellular populations.

• Utilized chromatographic techniques such as HPLC to analyze drug compounds.
• Analyzed in-vitro drug release characteristics and evaluation parameters for prepared formulations.
• Documented and evaluated test results using statistical analysis. Assisted scientists in method development and validation.

Development and Evaluation of sustained release tablets of propranolol HCl

• Applied characterization studies such as Fourier Transform Infrared (FTIR) Spectroscopy and Differential Scanning Calorimetry (DSC).
• In-vitro drug release characteristics and evaluation parameters for the prepared formulations.

• Handled laboratory analysis instruments like pH Meter, HPLC, UV-Vis, Dissolution Apparatus, and Viscosity Analyzer.
• Documenting and evaluating test results using statistical analysis. Maintained Lab records and assisted in quality assurance for the final batch record review as needed.