Denys Hudyma

During the university program get to acquaint with the lab basics and perform various experiments in multiple fields of biology This includes immunology( cell culture, flow cytometry, ELISA, cytotoxicity tests), histology(various tissue staining and examining), molecular biology(DNA isolation, ligation, protein isolation, purification, western blotting, enzyme activity assay), microbiology(bacteria(pathogenic) identification, bacterial cultures) and plant biology(growing on soil and in vivo, transformation of plant with bacteria(binary vector system) and further regeneration, modification with constructs by Golden Gate Modular Cloning, High-Performance Liquid Chromatography, phenotyping and genotyping(KASP analysis), RNA isolation and qPCR, protoplast transformation with polyethylene glycol)