Chaitrashree Nagabhushan

Performing research and projects oriented role.
- Strong pursuit in embryo rescue, organogenesis, mutant rescue, somatic embryogenesis in ornamental plants such as Poinsettia, Anthurium, Chrysanthemum and Petunia.
- Hands on experience in ploidy alterations, plant rejuvenation by meristem culture and pathogen elimination.
- Preparation and maintenance of all the necessary hormone stocks.
- Taken part in creating SOPs.
- Standardized accurate documentation and data recording helping in data analysis.
- Setting up experiments including researching about the instruments to be used in trials.
- Communication with breeders and stake holders about the findings from trials discussing the further steps or treatments.
-Keeping track of plants and samples incoming and arranging plant shipments to various locations in and out of Europe.

-Adapted quickly to new laboratory techniques and technologies as required.

- Adherence to laboratory safety with using necessary PPE, sterile working and safe disposing of chemicals.

- Initially taken part in various project later lead the projects.

Standardization of optimal conditions for fermentation process to reduce caffeine content in coffee waste from industries using fungal fermentation.
- Extensive literature review, scientific writing and creating SOP for fermentation process.
- Analysis of by-products (methylxanthines) using chromatographic techniques. And application of them in curing skin cancer.
-Standardized media composition and optimal growth conditions for culture and maintenance of murine melanoma cell lines.
- Hands on experience on cell culture assays such as MTT, DNA fragmentation assay, Cell via count, Flowcytometry, different staining techniques including immunostaining and florescence staining.
-Created SOP for flow cytometer handling for cell viability testing.
-Hands on experience in ELISA, Florescence microscopy, Gel electrophoresis.
-Trained and guided 2 masters intern starting from designing experiments, executing and approval of thesis.

Major responsibility on enhancing phytochemicals in callus of 2 underutilized fruits (Malpighia glabra and Ixora coccinia) using different plant tissue culture techniques.
- Standardized protocols for Phytoconstituent profiling of the fruit extract during different ripening stages of fruits.
- Enhancing, extraction and profiling of secondary metabolites from cell suspension cultures using chromatographic techniques.
- Hands on experience on Purple mutant carrot for enhanced production of anthocyanins.
- Callus and root and suspension cultures of Mulberry varieties.
-Standardization of product development using the 2 underutilized fruits for retaining the innate phytoconstituents: Microbial analysis, proximate analysis, phytoconstituent profiling.
- Scientific writing- Acquired 3 research articles, 2 are under communication.